T-cell proliferation and cell-cycle analyses. (A) PKH staining showing the number of duplications of T cells under stimulation with either phytohemagglutinin A or anti-CD3 plus anti-CD28. A representative case of 8 cases is shown. (B) Box plot showing the dose response effect of 5-azaC on T-cell proliferation. (C) Cell-cycle analysis in stimulated T cells in the absence (i) or presence (ii) of 5-azaC at 1μM (P = .01). A representative case of 8 cases is shown.