Effect of 5-azaC on the activation pattern of T cells. (A) Dot plot showing the forward scatter (FSC) and side scatter (SSC) of resting versus anti-CD3 plus anti-CD28 stimulated T cells. The addition of 5-azaC significantly inhibited the activation of T cells as shown by a lower FSC/SSC among stimulated 5-azaC–treated T cells as well as a lower number of T cells expressing CD25 and intracellular IFN-γ and CD40L. A representative case of 9 experiments is shown. (B) Box-plot showing the dose-dependent effect of 5-azaC on the different activation parameters analyzed. (C) Supernatants CBA cytokine assays after 4 days of culture. A representative case of 5 cases analyzed is shown.