OxPAPC has an impact on LPS-induced promoter modifications. DCs pretreated for 20 minutes with 10 μg/mL OxPAPC were stimulated with LPS (100 ng/mL) for 60 minutes and fixed with formaldehyde. Chromatin immunoprecipitation was performed with the use of an α-H3pS10, α-NF-κB p65, α-H3pS10/K14Ac, or an irrelevant antibody a control (unspecific). Immunoprecipitated DNA was analyzed by qPCR with promoter-specific primers for IL-12p40 (A), CD83 (B), and IκBα (C) and plotted as percent of input. Data are representative of 2 independent experiments.