OxPAPC differentially inhibits induction of activation-associated genes. DCs were stimulated with LPS (100 ng/mL) with or without pretreatment for 20 minutes with the indicated amounts of OxPAPC (5 or 10 μg/mL, LPS 5 and LPS 10, respectively) for 1 hour, and RNA was isolated and (A) IL-23p19, IL-12p35, IL-12p40, IκBα, and CD83 mRNA expression was determined by RT-PCR. (B) DCs were stimulated with LPS for 1 hour and subsequently treated with actinomycin D. They were then stimulated with the indicated amounts of OxPAPC for 1, 2, and 4 hours, and the abundance of the indicated mRNAs/glyceraldehyde-3-phosphate dehydrogenase (GAPDH) were determined by RT-PCR. Data are representative of 2 independent experiments.