Effect of mta3 knockdown on the expression of early mesoderm markers and hematopoietic precursor markers. (A-F) Embryos at the 1-cell stage were injected with 5 ng of cMO or tMO and examined for the expression of the indicated markers at indicated stages. Embryos at the shield stage or 75% epiboly stage are shown in lateral view; those at the 3-somite, 6-somite, or 10-somite stage were in posterodorsal view; and those at 24 hpf were in lateral view. Note that the expression of gata2 (E) and scl (F) was eliminated or dramatically decreased at all tested stages.