American Society of Hematology

Figure 3

$Figure 3. Asxl1tm1Bc/tm1Bc mutant mice exhibit reduced thymopoiesis. (A) Thymus cellularity is reduced in older (older than 15 weeks; group B, n = 8 each genotype) compared with younger (15 weeks or younger; group A, n = 6 wt, 4 mutant) Asxl1tm1Bc/tm1Bc (mut) adult mice compared with wild type. (B) Flow cytometry of T lymphocytes from adult thymus expressing the lineage markers CD4 and CD8 shows a relative increase in the double-negative and both single-positive fractions, and a relative decrease in the double-positive fraction in Asxl1tm1Bc/tm1Bc thymus compared with wild type. (C) Absolute cell number per thymus is reduced in group B Asxl1tm1Bc/tm1Bc mice compared with wild type for all fractions: (i) double-negative CD4−CD8− (DN), (ii) double-positive CD4+CD8+ (DP), (iii) single-positive (SP) CD4+, and (iv) single-positive CD8+ (n = 8). *P < .05.$

Asxl1^tm1Bc/tm1Bc mutant mice exhibit reduced thymopoiesis. (A) Thymus cellularity is reduced in older (older than 15 weeks; group B, n = 8 each genotype) compared with younger (15 weeks or younger; group A, n = 6 wt, 4 mutant) Asxl1^tm1Bc/tm1Bc (mut) adult mice compared with wild type. (B) Flow cytometry of T lymphocytes from adult thymus expressing the lineage markers CD4 and CD8 shows a relative increase in the double-negative and both single-positive fractions, and a relative decrease in the double-positive fraction in Asxl1^tm1Bc/tm1Bc thymus compared with wild type. (C) Absolute cell number per thymus is reduced in group B Asxl1^tm1Bc/tm1Bc mice compared with wild type for all fractions: (i) double-negative CD4⁻CD8⁻ (DN), (ii) double-positive CD4⁺CD8⁺ (DP), (iii) single-positive (SP) CD4⁺, and (iv) single-positive CD8⁺ (n = 8). *P < .05.

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