High risk and poor prognosis in MM is highly correlated with low expression of both JUN and EGR-1. JUN (A) and EGR-1 (C) gene expression levels (y-axis) in plasma cells from 98 subjects with MGUS, 104 with smoldering MM (SMM), and 544 newly diagnosed MM in the TT2 trial, as well as in 63 myeloma cell lines. The height of each bar indicates a sample's level of JUN (A) or EGR-1 (C) gene expression, as measured by Affymetrix microarray signal. Low JUN (B) and EGR-1 (D) expression levels were highly correlated with high-risk MM. JUN and EGR-1 expression levels, as measured by Affymetrix microarray signal, in 75 high-risk MM patients are significantly lower than in 469 low-risk MM patients. By the use of the optimal cutoffs, EFS (E, F, I, and J) and OS (G, H, K, and L), analyses were performed on JUN (E-H) or EGR-1 (I-L) mRNA expression levels, measured by Affymetrix microarray signal, in the TT2 and TT3 datasets.