Figure 3
Platelet GPVI and FXII activation contribute to collagen-promoted thrombin generation in mouse plasma. Thrombin generation was measured in PRP from wild-type mice that was treated with vehicle solvent (control) or collagen type I/H (5 μg/mL) and activated with CaCl2. Plasma samples were preincubated with FVIIai (100 nM), CTI (4 μM), and/or JAQ1 mAb (40 μg/mL). Thrombin generation was determined in the absence (A) or presence (B) of tissue factor (1 pM). Thrombin levels are given as arbitrary activity units (AAU). Note the suppression of collagen-dependent thrombin generation by JAQ1 mAb and CTI. Mean ± SEM; n = 4; *P < .05 vs control; #P < .05 vs condition with collagen.
