Evolution of chemokine receptor expression in R-sIL-7–treated macaques. (A) CXCR4, CCR7, CCR6, and CCR9 expression was FACS-quantified on circulating CD4⁺ and CD8⁺ T cells at H0, H6, and day 2 after R-sIL-7gly injection in healthy rhesus macaques (no. 14424, white symbols; no. 14694, black symbols). Representative FACS is shown for CD4 T cells (left histograms) and CD8 T cells (right histograms). Gray histograms represent chemokine receptor-negative cells. Percentages of chemokine receptor-positive cells are shown for each sample. (B) The evolution of CXCR4, CCR6, CCR7, and CCR9 expression on naive, TCM, and TEM CD4⁺ (left panels on each graph) and CD8⁺ (right panels) was measured by FACS analysis in R-sIL-7gly–injected healthy rhesus macaques (no. 14424, white symbols; no. 14694, black symbols). For each T-cell subset, chemokine expression was measured at H0, H6, and D2 (left to right).