Analysis of cell subsets in the spleen of DKO mice in steady state. (A) Total cellularity of spleens from WT, GM-CSF^−/−, FL^−/−, and DKO mice. Horizontal lines indicate mean values of the results. (B) Percentage of CD19⁺ and CD3⁺ cells in the spleen. (C) Percentage of myeloid cell subsets: Monocytes were first gated as M-CSFR⁺CD11b⁺ and separated into Gr1⁺ or Gr1⁻ cells; NK cells were gated as CD3⁻NK1.1⁺; DCs were divided into cDCs (MHCII⁺CD11c⁺) and pDCs (CD19⁻CD45RA⁺CD11c⁺) with representative FACS plots shown (D). CD45RA versus CD11c plots were first gated on CD19⁻ cells. Numbers in FACS plots are percentages of total nucleated cells. *P < .05; **P < .01; ***P < .001.