Flt3, Gm-csfr, and M-csfr mRNA expression in progenitor and myeloid cells. Real-time PCR analysis of mRNA expression of Flt3 (A), Gm-csfr (B), and M-csfr (C) in cell populations sorted from WT mice based on the following markers: c-kit^hi (lin⁻c-kit^hi); MDP^Δ (lin⁻M-CSFR^hi); CDP (lin⁻c-kit^intFlt3⁺IL7Rα⁻); spleen (Sp) and LN cDC (CD19⁻MHCII⁺CD11c⁺) and pDC (CD19⁻CD45RA⁺CD11c⁺); LN CD40^hi DC (CD19⁻CD40^hiCD11c^int); Langerhans cells (LC from epidermis) and dermal DC (MHCII⁺CD45⁺); blood (Bl), spleen, and bone marrow (BM) monocytes (mo) (M-CSFR⁺CD11b⁺ and Gr-1⁺ or Gr-1⁻). Data shown are averages of 3 independent experiments.