MAPC-mediated suppression in vitro is independent of Tregs. B6 > BALB/c MLR culture was performed using purified T cells or T cells that were CD25-depleted and MAPCs at 1:10 ratios. ³H-thymidine was added on the indicated days, and proliferation was measured (A). FACS analysis was performed on day 5 on the non-CD25–depleted (B) and the CD25-depleted (C) MLR cocultures to determine the percentage of CD4⁺FoxP3⁺ T cells.