Gene enrichment analysis to compare key genes up-regulated by hypoxia in human MDMs and known NF-κB–regulated genes. Hypoxia up-regulated genes identified in 2 separate macrophage cultures using Affymetrix or Illumina microarrays were ranked by level of hypoxia-mediated induction. The ranked gene lists were then compared with both a previously published gene set for hypoxia-regulated genes in tumor cells (the hypoxia-regulated gene set, top row) or genes previously shown to be NF-κB target genes (the NF-κB target gene set, bottom row) by GSEA. The hypoxia-regulated gene set (top row) was significantly enriched in the hypoxic macrophage gene set identified on both the Affymetrix (Array 1; NES = 2.2, q < 0.001) and the Illumina (Array 2; NES = 2.24, q < 0.001) arrays. The NF-κB target gene set (lower row) was also enriched in the hypoxic macrophage gene set on both Affymetrix (NES = 1.69, q = 0.02) and Illumina (NES = 1.67, q = 0.12) arrays.