KLRG1 inhibits adhesive properties on E-cadherin-expressing cells. (A) Parental or WT E-cadherin cells were stained with KLRG1 tetramer or E-cadherin antibody and analyzed by FACS. (B) Approximately 7.5 × 104 parental or WT E-cadherin–transduced cells were either treated with KLRG1 tetramer at the indicated concentrations or left untreated (1× HEPES) for 24 hours at 37°C. Fibroblast-like adhesive properties were imaged at ×20 with an Olympus DP70. Black arrowheads show fibroblast-like cells, and white arrowheads depict round circular cells. Data are representative of 3 independent experiments performed in a double-blinded manner. (C) Quantitative analysis of the experiments described in panel B. Three fields were analyzed, and between 50 and 200 cells were counted per field.
