Itk null CD4⁺ γδ T cells develop independent of the γδ TCR they express. (A) γδ T cells in the thymus and spleens of WT and Itk^−/− mice were analyzed for the expression of Vγ1.1 and Vδ6.2/3. Data are representative of 3 independent experiments. (B) γδ T cells in the thymus and spleens of KN6 transgenic mice, or KN6 transgenic mice lacking Itk, were analyzed for the expression of CD4 and CD8. Data are representative of 2 independent experiments.