Increased CD4⁺ γδ T cells in the absence of Itk is independent of MHC class I or II expression. (A) γδ T cells in the thymus and spleens of MHCI^−/−, MHCI/Itk DKO, MHCII^−/−, and MHCII/Itk DKO mice were analyzed for the expression of CD8 and CD4. Data are representative of 2 independent experiments. (B) The number of CD4⁺ γδ T cells in the mice analyzed in panel A were enumerated and presented (n = 3 mice). *P < .05.