Modulation of miR expression profile by TLR agonists. (A-C) MiR up- or down-modulated at least 1.5-fold in MD-DCs treated for 8 hours with R848 (2 μg/mL), LPS (100 ng/mL), or their combination with respect to control MD-DCs. Fold change (FC) was calculated dividing the normalized signals of treated samples by the control sample. P values, calculated by ANOVA, were less than .05 for all miR. (D) Dendrogram and heatmap of the modulated miR, shown in (A-C), obtained after cluster analysis. Clustered miR are listed on the right along with their P values. (E) Differentially expressed miR in MD-DCs stimulated with agonist combination versus cells treated with single agonists displayed as FC of expression. P values, calculated by ANOVA, were less than .05 for all miR.