In vivo Matrigel assay of angiogenesis in A fumigatus-infected and uninfected control mice. The antiangiogenic effects of A fumigatus in mice with cutaneous invasive aspergillosis were assessed using an in vivo Matrigel assay. In uninfected control mice, endothelial cell migration and capillary network formation occurred 5 days after implantation of the Matrigel; 7 days after implantation, erythrocyte-filled lacunae formed in the Matrigel. In contrast, angiogenesis was significantly suppressed in Matrigel plugs extracted from A fumigatus-infected mice 5 and 7 days after inoculation. Plugs obtained from ΔlaeA-infected mice exhibited endothelial cell infiltration similar to that of plugs obtained from uninfected control mice. Insets show enhanced details of endothelial networks. **P < .001. P values refer to the comparison with Matrigel plugs obtained from uninfected control mice and plugs from ΔlaeA-infected mice.