Figure 1
Effect of PAS on CLL cells. (A) Cells from CLL patients were treated with 0 to 160 μM PAS for 24 hours (n = 30), 48 hours (n = 9), or 72 hours (n = 10). Viability was assessed by MTT analysis. IC50 values were determined using CalcuSyn software as previously described.30 (B) Proteins were extracted from cells of patient AS15 incubated with no addition or the indicated concentration of PAS and analyzed by Western blotting. PARP cleavage data are typical of 23 and the caspase 3 data of 3 experiments. The percentages of apoptotic cells observed by morphologic analysis (see supplemental Figure 2) is indicated below the Western blots and are typical of 33 experiments.
