Fetal liver hematopoiesis in distal Runx1-deficient embryos. (A) FACS analysis of AA4.1 and c-Kit expression in E14.5 fetal livers (FL) isolated from distal^+/+ (wt), distal^+/− (het), and distal^−/− (ko) embryos. (B) Percentage of c-Kit⁺AA4.1⁺ population in wt (n = 4), het (n = 12), and ko (n = 6) fetal livers. Bars indicate average number for each group. (C) Number of hematopoietic colonies generated in methylcellulose from wt (n = 4), het (n = 12), and ko (n = 6) fetal livers. Bars indicate average number for each group.