Th17 cells and their associations with immune cell subsets. (A-D) The correlation between Th17 cells and T-cell subsets in the same tumor environment. Multiple tumor-infiltrating T-cell subsets were defined with specific staining and analyzed by FACS. The percentages of Th17 cells in CD4⁺ T cells, IFN-γ⁺CD8⁺ T cells in CD8⁺ T cells, and IFN-γ⁺IL-17⁺CD4⁺ T cells in IL-17⁺CD4⁺ T cells (Th17 cells) were quantified in tumor tissues. The correlations between the percentages of Th17 cells and IFN-γ⁺CD4⁺ T cells (A), IFN-γ⁺CD8⁺ (B), IFN-γ⁺IL-17⁺ T cells (C), and FOXP3⁺CD4⁺ T cells (D) were evaluated. Correlation coefficients were computed to assess the relationship between Th17 cells and T-cell subsets in the same tumor environments. (E) The relationship between Th17 and NK cells in the same tumor environment. Th17 cells and NK cells were defined with specific staining and analyzed by FACS. Results are expressed as the percentage of NK cells in CD45⁺ cells. NK cells were quantified as the percentage of CD16⁺CD56⁺ cells in CD45⁺ cells in tumor ascites by gating on CD45⁺, non-T, B, and myeloid cells. The samples were divided into 2 groups based on median percentage of Th17 cells.