![The synergy of RT plus ad-LIGHT immunotherapy eradicates distant metastases. 4T1 tumor: Balb/c mice were subcutaneously injected with 105 cells on the lower back. Mice received local RT (12 Gy) on days 14 and 15 and intratumoral injection with ad-control (2 × 1010 virus particles [vp]) or ad-LIGHT (2 × 1010 vp) on days 15 and 16 (n = 24-41 pooled from 5 experiments). B16-CCR7 tumor: C57BL/6 mice were subcutaneously injected with 105 cells on the lower back. Mice received local RT (12 Gy) on days 14 and 15 and intratumoral injection with ad-control (2 × 1010 vp) or ad-LIGHT (2 × 1010 vp) on days 15, 16, and 17 (n = 6-9 pooled from 2 experiments). On day 25 after tumor injection, tumors were surgically removed. Mice were killed on day 35 for tumor colonogenic assay (n = 4 or 5 per group). No colonies were detected in combination group in both types of tumor. Similar experiments were repeated 3 times.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/114/3/10.1182_blood-2009-02-206870/4/zh89990936940004.jpeg?Expires=1720860270&Signature=Eb4kKMy0yBVxJI6E-ekaYyWpc68BpOmlaMQQXxPvcQHBR9hca1XUKB2US0PGwIKUvC~khrF97ykpSmyH6Iq3Oh98vDH94E1lZP~GCCjeWJzUW3HTtIeE~QUjfLDVQpBzB204RRyfJ80qz7P3oYKlCz~0o~hhs~7qNdpikBF~cLlgb6Xqah5lQRfRf5dQMfDAvsvL7Xpid3bh3m-mN8X5QoEvGLmVVmqbAmISqIn8bs0Smkh5Nr2IC6prCr3V-69gjwBSyi1rwNufbiw4wPye1moSi7k5dbDVH-0fFWWIA16alMroc1nrHc3R~MO925CqtjCBB1gRB~UYlBIrYmdj0Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The synergy of RT plus ad-LIGHT immunotherapy eradicates distant metastases. 4T1 tumor: Balb/c mice were subcutaneously injected with 105 cells on the lower back. Mice received local RT (12 Gy) on days 14 and 15 and intratumoral injection with ad-control (2 × 1010 virus particles [vp]) or ad-LIGHT (2 × 1010 vp) on days 15 and 16 (n = 24-41 pooled from 5 experiments). B16-CCR7 tumor: C57BL/6 mice were subcutaneously injected with 105 cells on the lower back. Mice received local RT (12 Gy) on days 14 and 15 and intratumoral injection with ad-control (2 × 1010 vp) or ad-LIGHT (2 × 1010 vp) on days 15, 16, and 17 (n = 6-9 pooled from 2 experiments). On day 25 after tumor injection, tumors were surgically removed. Mice were killed on day 35 for tumor colonogenic assay (n = 4 or 5 per group). No colonies were detected in combination group in both types of tumor. Similar experiments were repeated 3 times.
