CALM-AF10 fusion protein causes H3-K79 hypomethylation. (A) H3K79 methylation in human T-REx-293 cell lines stably transfected with the indicated expression constructs. The cells were not induced (−) or induced with tetracycline for 48 hours (+), or analyzed after 72-hour further culturing after the withdrawal of tetracycline (lane 3). (B) H3K79 methylation in blast cells from leukemic mice with retroviral expression of CALM-AF10. Whole-cell extracts were prepared from leukemic cells with CALM-AF10 expression (right lane) and bone marrow transduced with the retroviral vector only expressing GFP (left lane), and were probed with indicated histone antibodies. All the cells were from GFP-positive sorting by FACS.