Figure 2
Figure 2. Effects of PKC412 on expression of Bim in Ton.Kit cells. (A) Ton.Kit.D816V cells were grown in control medium (control), or in the presence of doxycycline to induce KIT D816V expression. KIT D816V–positive cells were cultured in control medium (doxycycline) or in the presence of PKC412 (1μM) for 12 hours. Thereafter, cells were subjected to Western blotting performed with an antibody against Bim. The β-actin loading control is also shown. (B) Ton.Kit.wt cells were grown in control medium (control) or in the presence of doxycycline and stem cell factor (SCF; for induction of wt KIT). Wt KIT–positive cells were cultured in control medium (doxycycline + SCF) or in the presence of PKC412 (1μM) for 12 hours. Then, Western blotting was performed using antibodies against Bim and β-actin.

Effects of PKC412 on expression of Bim in Ton.Kit cells. (A) Ton.Kit.D816V cells were grown in control medium (control), or in the presence of doxycycline to induce KIT D816V expression. KIT D816V–positive cells were cultured in control medium (doxycycline) or in the presence of PKC412 (1μM) for 12 hours. Thereafter, cells were subjected to Western blotting performed with an antibody against Bim. The β-actin loading control is also shown. (B) Ton.Kit.wt cells were grown in control medium (control) or in the presence of doxycycline and stem cell factor (SCF; for induction of wt KIT). Wt KIT–positive cells were cultured in control medium (doxycycline + SCF) or in the presence of PKC412 (1μM) for 12 hours. Then, Western blotting was performed using antibodies against Bim and β-actin.

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