Effects of rhBAFF and/or BR3-Fc on depolarization of mitochondrial transmembrane potential (ΔΨm) in platelets in 9 ITP patients. (A) Platelets were gated by flow cytometry. (B-D) Representative flow cytometric dot plots in different groups. (C) Note that rhBAFF induces ΔΨ_m depolarization in platelets characterized by transformation of JC-1 dye aggregates to JC-1 monomers, which indicated that rhBAFF promoted early apoptosis of platelets. (D) BR3-Fc corrected the effects of rhBAFF on depolarization of ΔΨ_m in platelets. (E) The apoptosis of platelets by JC-1 in different groups. RhBAFF promoted the apoptosis of platelets and BR3-Fc corrected the effects of rhBAFF. Statistical significance was determined by analysis of variance. Bars represent SD; *P < .05.