RUNX1 binds transiently to Pu.1 in hemangioblasts. DamId assay analyzing GATC methylation levels at the indicated genes with hemangioblasts expressing Dam-methylase alone (iDam control) or RUNX1-Dam (iRUNX1-Dam) after a 4-hour induction with DOX. Differential methylation was detected by cleaving first with Dpn1, which cleaves only methylated DNA, and then with DpnII, which cleaves only unmethylated DNA.³²  The Chr2 control represents a nonexpressed GAPDH pseudogene. The values represent the mean values of 3 independent experiments.