SCS macrophages are not responsible for Ag transport to FDCs. (A) LN sections from WT mice were stained for B220 (white), FDC-M2 (blue), and MOMA-1 (red), then analyzed by confocal microscopy to highlight the SCS macrophages and their dendritic protrusions that penetrate the follicles. (B) WT mice were injected with 30 μL of PBS- or chlodornate-loaded liposomes in the hind footpads. Six days later, when SCS macrophage depletion was complete, 10 μL (50 μg) of Alexa 647–conjugated WGA was injected in the footpads of the treated animals. Popliteal LNs were harvested 30 minutes later; sectioned; stained for MOMA-1 (white), collagen IV (red), and FDC-M2 (blue); and then analyzed by confocal microscopy. Data were acquired with a Leica Sp5 microcope (×20 and ×63 objectives) and are representative of 2 different experiments. Scale bar: 100 μm.