Pomalidomide selectively activates Rho GTPases in primary human monocytes. (A) RhoA, Rac1, Cdc42, and Ras activities were measured by pull-down assay in primary monocytes in the presence of pomalidomide at indicated concentrations for 30 minutes. (B) Quantitation of GTPases activation in panel A using densitometry is expressed as percent control relative to DMSO treatment. (C-D) RhoA and Rac1 activity were determined by G-LISA assay in the presence of 1 μM pomalidomide at indicated time points. These results are representative of 3 independent experiments.