Immunoblot analysis of potential target proteins in CLL primary cells treated with SGI-1776. CLL primary cells (patient no. 5) were either untreated, treated with 0.1% vehicle DMSO alone, or treated with 3 or 10 μmol/L SGI-1776, and cells were harvested after 2, 4, 8, and 24 hours and lysed. The protein levels of pBad(Ser112), total Bad, histone H3(Ser10), total histone H3, and p53 were analyzed by the use of immunoblot and normalized with GAPDH as a loading control.