Figure 7
Neutrophil-specific deletion of Syk results in a defective host response to S aureus. Analysis of syk deletion by flow cytometry of Syk protein in (A) neutrophils (Ly6G+CD11b+) and (B) monocytes (Ly6G−CD11b+) from the bone marrow (BM), peripheral blood (PB), and air pouch lavage (AP). SykKO cells from syk−/− chimeras or syk f/fVav1creTg mice are negative controls (gray histograms) relative to sykf/−LysMcreTg/+, sykf/−Mrp8creTg (dashed black lines), or sykf/− (solid black line) mice. Histograms are representative of at least 4 animals. (C) CFU of S aureus and neutrophil infiltrate and (D) CFU of E coli and neutrophil infiltrate in the air pouch lavage of sykf/+LysMcreTg/+ and sykf/−LysMcreTg/+ mice 24 hours after the initiation of infection. (E) CFU of S aureus and neutrophil infiltrate and (F) CFU of E coli and neutrophil infiltrate in the air pouch lavage of sykf/f, sykf/−, and sykf/−Mrp8creTg mice 24 hours after the initiation of infection. Data represent at least 3 independent experiments, with at least 3 animals per group. The difference in S aureus counts in the air pouch is statistically significant; *P < .05 by Mann-Whitney U test. (G) Analysis of TNF-α and MIP-1α levels in S aureus–infected air pouches from sykf/+LysMcreTg/+ and sykf/−LysMcreTg/+ mice. *P < .05, unpaired t test.

Neutrophil-specific deletion of Syk results in a defective host response to S aureus. Analysis of syk deletion by flow cytometry of Syk protein in (A) neutrophils (Ly6G+CD11b+) and (B) monocytes (Ly6G−CD11b+) from the bone marrow (BM), peripheral blood (PB), and air pouch lavage (AP). SykKO cells from syk−/− chimeras or syk f/fVav1creTg mice are negative controls (gray histograms) relative to sykf/−LysMcreTg/+, sykf/−Mrp8creTg (dashed black lines), or sykf/− (solid black line) mice. Histograms are representative of at least 4 animals. (C) CFU of S aureus and neutrophil infiltrate and (D) CFU of E coli and neutrophil infiltrate in the air pouch lavage of sykf/+LysMcreTg/+ and sykf/−LysMcreTg/+ mice 24 hours after the initiation of infection. (E) CFU of S aureus and neutrophil infiltrate and (F) CFU of E coli and neutrophil infiltrate in the air pouch lavage of sykf/f, sykf/−, and sykf/−Mrp8creTg mice 24 hours after the initiation of infection. Data represent at least 3 independent experiments, with at least 3 animals per group. The difference in S aureus counts in the air pouch is statistically significant; *P < .05 by Mann-Whitney U test. (G) Analysis of TNF-α and MIP-1α levels in S aureus–infected air pouches from sykf/+LysMcreTg/+ and sykf/−LysMcreTg/+ mice. *P < .05, unpaired t test.

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