American Society of Hematology

Figure 6

$Figure 6. ESC-HSC are CD150+ and heterogeneous with respect to CD48 cell-surface expression. (A) CD48+ and CD48− EPOCH cells were isolated by magnetic bead selection and analyzed by flow cytometry before and after fractionation. (B) Kaplan-Meier survival analyses and (C) EPOCH cell-derived PB reconstitution of Rag-2−/−γc−/− mice transplanted with CD48 EPOCH cells fractions. EPOCH cell-derived PB is GFP+. Data are pooled from 2 experiments. (D) CD48−, CD48med, and CD48+ CD41high CD150+ EPOCH cells were isolated via FACS. A representative before- and after-sort analysis is shown. (E) Kaplan-Meier survival analyses and (F) EPOCH cell-derived PB reconstitution of Rag-2−/−γc−/− mice transplanted with CD41high EPOCH cells fractionated for CD150 and CD48 expression are shown. Data are pooled from 4 experiments. Error bars denote standard deviation.$

ESC-HSC are CD150⁺ and heterogeneous with respect to CD48 cell-surface expression. (A) CD48⁺ and CD48⁻ EPOCH cells were isolated by magnetic bead selection and analyzed by flow cytometry before and after fractionation. (B) Kaplan-Meier survival analyses and (C) EPOCH cell-derived PB reconstitution of Rag-2^−/−γc^−/− mice transplanted with CD48 EPOCH cells fractions. EPOCH cell-derived PB is GFP⁺. Data are pooled from 2 experiments. (D) CD48⁻, CD48^med, and CD48⁺ CD41^high CD150⁺ EPOCH cells were isolated via FACS. A representative before- and after-sort analysis is shown. (E) Kaplan-Meier survival analyses and (F) EPOCH cell-derived PB reconstitution of Rag-2^−/−γc^−/− mice transplanted with CD41^high EPOCH cells fractionated for CD150 and CD48 expression are shown. Data are pooled from 4 experiments. Error bars denote standard deviation.

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