ESC-HSC are CD41^highCD34⁻ and heterogeneous with respect to CD45 cell-surface expression. (A) CD41 and CD45 expression on EPOCH cells is shown. (B) Kaplan-Meier survival analyses and (C) EPOCH cell-derived PB reconstitution of Rag-2^−/−γc^−/− mice transplanted with EPOCH cells fractionated for CD41 or CD45 expression. EPOCH cell-derived PB is GFP⁺. Two independent experiments are presented. (D) CD45 and CD41 coexpression on EPOCH cells are shown. (E) Shown are Kaplan-Meier survival analyses and (F) EPOCH cell-derived PB reconstitution of Rag-2^−/−γc^−/− mice undergoing transplantation with EPOCH cells fractionated for both CD41 and CD45 expression. Two independent experiments are presented. Error bars denote standard deviation. (G) CD41 and CD34 expression on EPOCH cells. (H) Kaplan-Meier survival analyses and (I) EPOCH cell-derived PB reconstitution of Rag-2^−/−γc^−/− mice undergoing transplantation with EPOCH cells fractionated for CD34 expression are shown. Data in panel H summarize 4 experiments. Data in (I) summarize 7 CD34⁻ recipients.