Src is required for β-catenin–dependent NK-cell survival maintained by fibronectin. (A) The activation and expression of Src and β-catenin in NK cells cultured in the presence of fibronectin were analyzed by Western blot. (B) Expression of p-β-catenin of S675 and Y654 in NK cells cultured in the presence of fibronectin with or without pretreatment of PP2. (C) Nuclear translocation of β-catenin in NK cells cultured in the presence of fibronectin with or without pretreatment of PP2 was given by confocal imaging. Objective 63×, numeric aperture 1.4. (D) Viability of NK cells cultured in the presence of fibronectin with or without pretreatment of PP2 for 24 hours was tested using FACS. Data are shown as 1 typical result from 3 independent experiments with similar results or as mean ± SD of 3 independent experiments. *P < .05.