Src directly interacts with β-catenin. (A) Src was associated with β-catenin. HEK293 cells were transfected with PCDNA3.0 construct (-B), β-catenin construct (β), or β-catenin construct plus Src construct (β+S), respectively. The proteins were immunoprecipitated with anti–β-catenin antibody and blotted with anti-Src antibody. Protein in whole-cell lysate (W) was used as positive control. (B) Sorted NK cells cultured in the presence of fibronectin were collected at the indicated times. The proteins were immunoprecipitated with anti–β-catenin antibody and blotted with anti-Src antibody. (C) Example profiles of the fluorescence of donor (red) and acceptor (green) before and after photobleaching. The area circled by different color line was ROI. The Fret efficiency of the different ROI was shown in the histograph below the profiles. Objective 63×, numeric aperture 1.4. (D) The interaction of β-catenin and Src was testified by c-Fret technology in mouse NK cells after fibronectin treatment. The Fret efficiency of ROI of NK cells was dotted. Data are shown as 1 typical result from 3 independent experiments with similar results.