Fibronectin/CD11b maintains NK-cell survival via β-catenin. (A-C) The percentage (A) and number (C) of NK cells and CD11b⁺ NK cells (D) in spleen and the total number of splenocytes (B) of littermate control or β-catenin^−/− mice were analyzed by FACS. WT and β-catenin^{floxed/floxed} (β-catenin^fl/fl) mice were used as control mice. (E) The same number (2 × 10⁶) of CFSE-labeled NK cells sorted from β-catenin^{floxed/floxed} mice and PKH-26–labeled NK cells sorted from β-catenin^−/− mice were transferred to the wild-type mice. The CFSE⁺ or PKH-26⁺ NK1.1⁺ NK cells in 150 μL peripheral blood of the recipient mice were analyzed at the indicated times and counted using FACS. (F) The viability of NK cells derived from littermate control or β-catenin^−/− mice after culture in the presence of fibronectin was tested with FACS. Data are shown as 1 typical result from 3 independent experiments with similar results or as mean ± SD of 3 independent experiments. *P < .05; **P < .01.