The E-cadherin/catenin complex is induced in peritoneal alternatively activated macrophages elicited during T crassiceps infection. PEMs were isolated from BALB/c WT, IL-4^−/−, IL-4Rα^−/−, and STAT6^−/− uninfected mice or 8 and 12 weeks after T crassiceps infection. (A) The fold Cdh1 induction relative to the expression in PEMs from the corresponding uninfected mice (= 1) is shown. (B) Peritoneal macrophages were analyzed by immunoblotting using antibodies against E-cadherin, β-, α- or p120-catenin. β-Actin was applied as a loading control. (C) Immunofluorescence microscopy on peritoneal macrophages isolated from T crassiceps infected WT BALB/c mice (8 weeks after infection). PEMs were grown on glass coverslips and after E-cadherin (i), β-catenin (ii,v), or p120-catenin (iv) labeling; images were obtained using an Olympus CellM fluorescence microscope. Subpanels iii and vi are merged pictures of the respective green and red images. Scale bars represent 20 μm.