Relative abundance of unique TCRβ CDR3 sequences correlates inversely with divergence from germline. (A) Observed frequency (top panels) and average observed frequency (bottom panels) of TCRβ CDR3 sequences in the CD8⁺CD45RO^+/− and CD4⁺CD45RO^+/− T-cell compartments of 2 male donors plotted, from left to right, according to their Jβ and Vβ gene segment use, CDR3 length, and total number of (inserted + deleted) nucleotides at the Vβ-Dβ and Dβ-Jβ junctions. (B) Heat map representation of relative abundance of TCRβ CDR3 sequences observed in CD4⁺ naive, CD4⁺ memory, and CD8⁺ naive T-cell compartments of the 2 male donors arrayed according to the number of nucleotides deleted or inserted at the Vβ-Dβ and Dβ-Jβ junctions. Color indicates the log₁₀(observed frequency) of the sequences with the indicated number of inserted or deleted junctional nucleotides.