Migration of leukocytes to sites of inflammation in mice is impaired by blockage of PV-1. (A) Total number of leukocytes in peritoneal lavages. Peritonitis was induced by intraperitoneal injection of proteose-peptone + IL-1β. One hour later, antibodies were injected to the tail vein and peritoneal lavages collected after 18 hours. Cells of mice without inflammation have been also counted and set as 100%. (B) Analysis of leukocyte subsets in the peritoneal lavages. *P ≤ .05, **P ≤ .01, ***P ≤ .001. N = 11 for negative control–treated mice, n = 13 for PV-1–treated mice, and n = 6 for mice without inflammation. (C) Total numbers of leukocytes retrieved from the air pouches 18 hours after induction of inflammation by injection of CCL-21 and bovine serum albumin. Values of negative controls were set as 100%. (D) Analysis of leukocyte subpopulations. N = 6 for negative control (antiendoglin mAb)–treated mice and n = 8 for anti–PV-1 mAb–treated mice. Bars represent mean ± SEM.