Figure 5
Leukocyte adhesion was impaired in the absence of mAbp1 under flow conditions in vivo. Adhesion of mAbp1+/+ and mAbp1−/− PMNs under static conditions on immobilized (A) fibrinogen or (B) ICAM-1 30 minutes after treatment with TNFα (100 ng/mL) or Mn2+ (1 mM). Data represent adhesion in percentage of cells adherent to poly-l-lysine (100%). Means ± SDs; mAbp1+/+, n = 5; mAbp1−/−, n = 4; *P < .05 versus unstimulated control; n.s. indicates not significant. Intravital microscopic analysis of PMNs (C) rolling (leukocyte rolling flux fraction; mean percentage ± SEM) and (D) adhesion (means of adherent cells/mm2 ± SEMs) in TNFα-stimulated cremaster muscle venules of mAbp1+/+ (□) and mAbp1−/− mice (▩). (E) Cumulative frequency distribution of leukocyte rolling velocities in mAbp1+/+ mice (black line) and mAbp1−/− (gray line). (F) Quantitative analysis of intravascular and perivascular leukocytes in whole-mount TNFα-treated cremaster muscle preparations from mAbp1+/+ mice (□) and mAbp1−/− (▩). Means of cells/mm2 ± SEMs; n = 5; *P < .05.

Leukocyte adhesion was impaired in the absence of mAbp1 under flow conditions in vivo. Adhesion of mAbp1+/+ and mAbp1−/− PMNs under static conditions on immobilized (A) fibrinogen or (B) ICAM-1 30 minutes after treatment with TNFα (100 ng/mL) or Mn2+ (1 mM). Data represent adhesion in percentage of cells adherent to poly-l-lysine (100%). Means ± SDs; mAbp1+/+, n = 5; mAbp1−/−, n = 4; *P < .05 versus unstimulated control; n.s. indicates not significant. Intravital microscopic analysis of PMNs (C) rolling (leukocyte rolling flux fraction; mean percentage ± SEM) and (D) adhesion (means of adherent cells/mm2 ± SEMs) in TNFα-stimulated cremaster muscle venules of mAbp1+/+ (□) and mAbp1−/− mice (▩). (E) Cumulative frequency distribution of leukocyte rolling velocities in mAbp1+/+ mice (black line) and mAbp1−/− (gray line). (F) Quantitative analysis of intravascular and perivascular leukocytes in whole-mount TNFα-treated cremaster muscle preparations from mAbp1+/+ mice (□) and mAbp1−/− (▩). Means of cells/mm2 ± SEMs; n = 5; *P < .05.

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