Figure 2
No effect of mAbp1 on β2 integrin expression, up-regulation, and localization during phagocytosis or fibrinogen binding. (A) Flow cytometric analysis of cell-surface expression of GR-1, CD11b, and CD18 in mAbp1+/+ or mAbp1−/− PMNs stimulated with KC (100 ng/mL), TNFα (100 ng/mL), or left unstimulated. (B) Confocal microscopy images of murine PMNs phagocytosing serum-opsonized Alexa 594–conjugated E coli (red). Translocation of CD18 (green) to the site of particle binding (arrows) was not affected in Abp1−/− PMNs. Bar = 10 μm. Data are representative for 3 independent experiments. (C) Binding of Alexa 488–conjugated fibrinogen to murine mAbp1+/+ and mAbp1−/− PMNs as measured by flow cytometry. Cells were incubated for 20 minutes with EDTA as negative control, Mn2+ (3 mM), TNFα (100 ng/mL), or left unstimulated. Addition of 1 mM Mn2+ gave similar results (data not shown). Mean fluorescence intensity within marker 1 (M1) ± SD; n = 4; #P < .05 versus unstimulated control; n.s. indicates not significant versus wild-type control.

No effect of mAbp1 on β2 integrin expression, up-regulation, and localization during phagocytosis or fibrinogen binding. (A) Flow cytometric analysis of cell-surface expression of GR-1, CD11b, and CD18 in mAbp1+/+ or mAbp1−/− PMNs stimulated with KC (100 ng/mL), TNFα (100 ng/mL), or left unstimulated. (B) Confocal microscopy images of murine PMNs phagocytosing serum-opsonized Alexa 594–conjugated E coli (red). Translocation of CD18 (green) to the site of particle binding (arrows) was not affected in Abp1−/− PMNs. Bar = 10 μm. Data are representative for 3 independent experiments. (C) Binding of Alexa 488–conjugated fibrinogen to murine mAbp1+/+ and mAbp1−/− PMNs as measured by flow cytometry. Cells were incubated for 20 minutes with EDTA as negative control, Mn2+ (3 mM), TNFα (100 ng/mL), or left unstimulated. Addition of 1 mM Mn2+ gave similar results (data not shown). Mean fluorescence intensity within marker 1 (M1) ± SD; n = 4; #P < .05 versus unstimulated control; n.s. indicates not significant versus wild-type control.

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