TAPP2, utrophin, and syntrophin are coexpressed in freshly isolated B chronic lymphocytic leukemia cells. B-CLL cells were isolated, fixed, and centrifuged onto glass slides. (A) Samples were stained with rabbit anti-TAPP2, followed by Alexa 488 anti–rabbit secondary antibodies. Note the membrane/cytoplasmic staining of TAPP2 is variable from patient to patient (3 representative patients are shown). (B) Coexpression of utrophin and syntrophin in B-CLL. In the righthand panels, yellow color indicates colocalization of utrophin and syntrophin staining, and blue indicates nuclear staining with DAPI. Two independent patient samples are shown in top and bottom panels. No significant signal was seen when primary antibodies were omitted (controls not shown).