Effects of Hif-2α deletion on vessel permeability. (A) Increased vessel permeability in KO mice. Images of chronic vascular leakage in lung and adipose tissue of KO mice. (B) Quantification of Evan blue dye leakage in various organs. (C) Photographs of Evan blue dye leakage 30 minutes after intradermal injection of either PBS or VEGF into the back skin of 9-month-old control and KO mice. (D) Quantification of extracted dye from the back skin of 3-, 6-, and 9-month-old mice, normalized to tissue weight, showed significantly increased leakage in KO compared with control mice in both treatment groups (n = 8 for each age). **P < .01; *P < .05.