Endorepellin modulates downstream VEGF/VEGFR2 signaling by engaging SHP-1. (A) Serum-starved HUVECs grown on collagen were stimulated with human recombinant VEGF₁₆₅ (10 ng/mL) and endorepellin (25nM) either alone or in combination for 5 minutes as marked. When indicated, the cells were pretreated for 1 hour with Na₃VO₄ (1 μM) or NSC-87877 (1μM). Whole-cell lysates were analyzed on immunoblots for phospho-PLCγ1 or total PLCγ1 content. (B) Quantification of phospho-PLCγ1 normalized on total PLCγ1. Values represent means ± SEMs (n = 3); ***P < .001.