Endorepellin provokes dephosphorylation of multiple RTKs through the integrin α2β1 receptor. (A) Antibody array recognizing 42 distinct RTKs incubated with HUVEC extracts from cells maintained for 5 and 30 minutes in the absence or presence of endorepellin (100 nM) and probed with anti–phosphotyrosine antibody. HUVECs grown on collagen type I were serum-starved for 1 hour before the addition of endorepellin. The major affected RTKs are labeled and indicated by arrows. (B) Experiment as in panel A, with the exception of that the lower panel was preincubated for 1 hour with 10 μg/mL integrin α2 blocking antibody 1998z prior endorepellin stimulation (100nM) for 5 minutes. Different exposures of the membranes (2 and 5 minutes) are shown for easier visualization of changes. Control represents extract from untreated serum-starved HUVECs.