CD4^dimCD8^bright T cells are the polyfunctional CD8⁺ T-cell population. (A) Representative dot plots for evaluation of polyfunctional responses. The gating strategy in Figure 1A through F was used to arrive at the cumulative data shown here. (B-C) Cumulative data showing effector molecule production on respective antigen priming of CD4⁻CD8⁺ and CD4^dimCD8^bright T cells from HIV⁺ LTNP (B) and CMV⁺ (C) donors. The x-axis lists the effector molecule or combination produced (production is denoted by +). Responses are grouped and color-coded by the number of effector molecules produced. (D-E) Cumulative data showing the mean percentage of cells producing the respective number of effector molecules with no treatment (UNT), respective peptide treatment (PEP) or SEB treatment. Percentages listed denote total percentage of cells producing one or more molecules (sum of blue, red, green, and orange pies). (F-G) Cumulative data of mean percentage of expression of 1, 2, 3, or 4 molecules in response to HIV(F) or CMV (G) peptide stimulation. P values denote differences between CD4⁻CD8⁺ and CD4^dimCD8^bright T-cell cytokine expression as calculated using paired t test analysis.