Cooperative effect of miR-128b and miR-221 on restoration of glucocorticoid sensitivity. (A) Schematic of the constructs coexpressing wild-type and mutant versions of miR-128b and miR-221. Green and orange lines represent miR-221_mut or miR-128_mut and its complement, respectively. (B) Expression levels of mature miR-221 (left panel) and miR-128b (right panel) in sorted GFP⁺ cells that are overexpressing a construct expressing just miR-221 (221) or miR-128b (128b), or the double expression constructs miR-221–miR-128b (221-128b), miR-221–miR-128b_mut (221-128b_mut), or miR-221_mut–miR-128b (221_mut-128b). Control cells express the vacant vector. (C) Sorted GFP⁺ cells expressing just miR-128b or the double expression constructs miR-221–miR-128b, miR-221–miR-128b_mut, miR-221_mut–miR-128b, or miR-221_mut–miR-128b_mut, or control (vacant vector) were treated with different concentrations of DEX for 40 hours, followed by FACS analysis as in Figure 1. The vertical axis is plotted as in Figure 1D. Three independent experiments were done. **P < .05, miR-221-miR-128b versus all other constructs, and miR-221_mut–miR-128b versus miR-221_mut-miR–128b_mut. *P < .05, miR-221–miR-128b versus all other constructs except for miR-128b, and miR-221_mut–miR-128b versus miR-221_mut–miR-128b_mut.