Ectopic expression of miR-128b and miR-221 restores glucocorticoid sensitivity to SEM cells. (A) Sorted GFP⁺ control– (termed MDH), let7b–, miR-221–, or miR-128b–transduced cells are treated (DEX⁺) or not (DEX⁻) with 100 μM DEX for 40 hours, followed by FACS analysis. As in Figure 1, the percentage of viable cells is determined by dividing the number of annexin V⁻ GFP⁺ cells by the number of total cells analyzed (n = 4). **P < .05. (B) The cells were stained with propidium iodide (PI), and the percentage of viable cells is determined by dividing the number of PI⁻ GFP⁺ cells by the number of total cells analyzed (n = 4). **P < .05. (C) The absolute cell number of annexin V⁻ GFP⁺ cells before treatment (left panel), without (middle panel), or with 100 μM DEX treatment (right panel) for 40 hours. (n = 4). **P < .05. (D) Representative FACS profiles of the sorted control–, let7b–, miR-128b–, or miR-221–transduced RS4;11 cells with (DEX⁺) or without (DEX⁻) treatment with 100 μM DEX. The GFP⁻ cells in all panels represent dead cells in which GFP has been released; the majority are positive for annexin V staining. The percentage of viable cells is determined by dividing the number of annexin V⁻ GFP⁺ cells by the number of total cells analyzed.