Altered expression of the T-cell synapse cytolytic effector molecule Rab27A is associated with patient prognosis. (A) Representative high-power (× 40 magnification) images of Rab27A intrafollicular expression in CD3⁺ T cells on TMA cores from extremes of survival diagnostic FL biopsies (< 5 years [y] [short-survival group] and > 15 years [long-survival group]). Dot plot charts show significantly reduced intrafollicular Rab27A expression (mean intensity, left chart) and percentage of positive cells (right chart) in short-survival group. Statistical analysis was performed using a mixed effect linear model (P values shown). Intrafollicular and interfollicular IHC quantitative analysis for Itk, Filamin-A, and Rab27A is shown in supplemental Figures 2–4. (B) Overall survival by Rab27A expression on tissue microarray of previously untreated FL.