Figure 3
Figure 3. Only CD7+CD56+ cells express typical NK-cell markers, whereas CD7negCD56+ cells express myeloid-associated receptors. (A-K) CD3negCD14negCD19neg cells were gated for CD56 expression and CD56+ cells were plotted against CD7 to identify CD7+ NK cells and CD7neg monocyte/DC-like cells. CD7+CD56+ NK cells were further subset into CD4+ NK cells and CD4neg NK cells. The percentage of cells expressing each receptor was determined on each subset of cells. (L-M) Percentage of cells expressing CXCR3 (L) and mean fluorescent intensity (MFI; M) of GITR were assessed on CD4neg and CD4+CD7+ NK cells. (N-Q) Assessment of DC and monocytic markers on CD7+CD56+ cells and CD7negCD56+ cells. *P < .05. P values in panels A through K were determined between CD4negCD7+ NK cells and CD7neg monocyte/DC-like cells and between CD4+CD7+ NK cells and CD7neg monocyte/DC-like cells (n = 6).

Only CD7+CD56+ cells express typical NK-cell markers, whereas CD7negCD56+ cells express myeloid-associated receptors. (A-K) CD3negCD14negCD19neg cells were gated for CD56 expression and CD56+ cells were plotted against CD7 to identify CD7+ NK cells and CD7neg monocyte/DC-like cells. CD7+CD56+ NK cells were further subset into CD4+ NK cells and CD4neg NK cells. The percentage of cells expressing each receptor was determined on each subset of cells. (L-M) Percentage of cells expressing CXCR3 (L) and mean fluorescent intensity (MFI; M) of GITR were assessed on CD4neg and CD4+CD7+ NK cells. (N-Q) Assessment of DC and monocytic markers on CD7+CD56+ cells and CD7negCD56+ cells. *P < .05. P values in panels A through K were determined between CD4negCD7+ NK cells and CD7neg monocyte/DC-like cells and between CD4+CD7+ NK cells and CD7neg monocyte/DC-like cells (n = 6).

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