Systemic PGE₂ treatment selectively expands CD150⁺ CD48⁻ LSK cells. (A) Flow cytometric dot plot illustrating gating scheme for subsets of LSK cells using SLAM receptors. Parent population is Lin⁻ Sca1⁺ c-Kit⁺. (B-D) Flow cytometric analysis of the frequency of LSK cells (B), LT-HSCs (C), and ST-HSCs/MPPs (D) in the bone marrow after PGE₂ or vehicle treatment. *P ≤ .05 (3 separate experiments are included).